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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD151 Polyclonal antibody (27816-1-AP)

    WB analysis using 27816-1-AP (same clone as 27816-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27816-1-AP (CD151 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27816-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CD151 Polyclonal antibody (27816-1-AP)

    IHC staining of human ovary tumor using 27816-1-AP (same clone as 27816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 27816-1-AP (CD151 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27816-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CD151 Polyclonal antibody (27816-1-AP)

    IHC staining of human ovary tumor using 27816-1-AP (same clone as 27816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 27816-1-AP (CD151 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27816-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CD151 Polyclonal antibody (27816-1-AP)

    IHC staining of human stomach cancer using 27816-1-AP (same clone as 27816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27816-1-AP (CD151 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27816-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CD151 Polyclonal antibody (27816-1-AP)

    IHC staining of human stomach cancer using 27816-1-AP (same clone as 27816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27816-1-AP (CD151 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27816-1-PBS in a different storage buffer formulation.

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CD151 Polyclonal antibody, PBS Only

CD151 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27816-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27816-1-PBS targets CD151 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD151 fusion protein Ag25968 相同性解析による交差性が予測される生物種
Full Name CD151 molecule (Raph blood group)
Calculated molecular weight 28 kDa
Observed molecular weight30 kDa
GenBank accession numberBC001374
Gene Symbol CD151
Gene ID (NCBI) 977
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48509
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD151 (also known as TSPAN24) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are involved in the regulation of cell development, activation, growth and motility. CD151 is broadly expressed by a variety of cell types. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. CD151 enhances cell motility, invasion and metastasis of cancer cells.