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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PE Anti-Mouse CD16 / CD32 (2.4G2)

CD16 / CD32 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

2.4G2

Cat no : PE-65080

Synonyms

AI528646, CD16 / CD32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using PE Anti-Mouse CD16 / CD32 (2.4G2) (PE-65080)

    FC experiment of mouse splenocytes using PE-65080

    1X10^6 C57BL/6 mouse splenocytes were surface stained with APC-Anti-Mouse CD3ε (APC-65060, Clone: 145-2C11) and 0.13 ug PE Anti-Mouse CD16 / CD32 (PE-65080, Clone: 2.4G2). Cells were not fixed.

  • Flow cytometry (FC) experiment of mouse splenocytes using PE Anti-Mouse CD16 / CD32 (2.4G2) (PE-65080)

    FC experiment of mouse splenocytes using PE-65080

    1X10^6 C57BL/6 mouse splenocytes were surface stained with APC-Anti-Mouse CD3ε (APC-65060, Clone: 145-2C11) and 0.13 ug PE-Rat IgG2b isotype control antibody. Cells were not fixed.

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Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65080 targets CD16 / CD32 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name Fc receptor, IgG, low affinity IIb
GenBank accession numberBC038070
Gene symbol CD16/32
Gene ID (NCBI) 14130
RRIDAB_2883876
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. The 2.4G2 antibody reacts with the extracellular domains of mouse CD16 (FcRIII) and CD32 (FcRII) (PMID: 90108; 8406898).

Protocols

Product Specific Protocols
FC protocol for PE CD16 / CD32 antibody PE-65080Download protocol
Standard Protocols
Click here to view our Standard Protocols