Validation Data Gallery
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Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
APC-65057 targets CD16/32 in FC applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, lambda |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | VH81X Tg B cells and T220 stromal cell line 相同性解析による交差性が予測される生物種 |
| Full Name | Fc receptor, IgG, low affinity IIb |
| GenBank accession number | BC038070 |
| Gene Symbol | CD16/32 |
| Gene ID (NCBI) | 14130 |
| RRID | AB_2882959 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 650 nm / 660 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P08101 |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for APC CD16/32 antibody APC-65057 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |