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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PE Anti-Human CD16 (3G8)

CD16 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

3G8

Cat no : PE-65090

Synonyms

Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen, CD16A

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of human PBMCs using PE Anti-Human CD16 (3G8) (PE-65090)

    FC experiment of human PBMCs using PE-65090

    1x10^6 human PBMCs were surface stained with APC Anti-Human CD3, and 5 ul PE Anti-Human CD16 (3G8) (PE-65090, Clone:3G8) or PE Mouse IgG1 Isotype Control (MOPC-21) (PE-65124, Clone: MOPC-21). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using PE Anti-Human CD16 (3G8) (PE-65090)

    FC experiment of human PBMCs using PE-65090

    1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD16 (3G8) (PE-65090, Clone:3G8) (red) or PE Mouse IgG1 Isotype Control (MOPC-21) (PE-65124, Clone: MOPC-21) (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 1 publications below

Product Information

PE-65090 targets CD16 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human PMN cells 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Calculated molecular weight 254 aa, 29 kDa
GenBank accession numberBC017865
Gene symbol CD16a
Gene ID (NCBI) 2214
RRIDAB_2883884
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).

Protocols

Product Specific Protocols
FC protocol for PE CD16 antibody PE-65090Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

J Bone Miner Res

Single-cell RNA sequencing reveals that an imbalance in monocyte subsets rather than changes in gene expression patterns is a feature of postmenopausal osteoporosis

Authors - Lin Tao
View Article