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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD163 (GHI/61)

CD163 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

GHI/61

Cat no : 65169-1-Ig

Synonyms

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD163 (GHI/61) (65169-1-Ig)

    FC experiment of human PBMCs using 65169-1-Ig

    1X10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD163 (65169-1-Ig, Clone:GHI/61) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red) or unstained. Cells were not fixed. Granulocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD163 (GHI/61) (65169-1-Ig)

    FC experiment of human PBMCs using 65169-1-Ig

    1X10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD163 (65169-1-Ig, Clone:GHI/61) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Granulocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65169-1-Ig targets CD163 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Glycoprotein preparation from hairy cell leukemia spleen 相同性解析による交差性が予測される生物種
Full Name CD163 molecule
Calculated molecular weight 1156 aa, 125 kDa
GenBank accession numberBC051281
Gene symbol CD163
Gene ID (NCBI) 9332
RRIDAB_2918446
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

Protocols

Product Specific Protocols
FC protocol for CD163 antibody 65169-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols