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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 555 Anti-Human CD163 (GHI/61)

CD163 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

GHI/61

Cat no : CL555-65169

Synonyms

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 555 Anti-Human CD163 (GHI/61) (CL555-65169)

    FC experiment of human PBMCs using CL555-65169

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD163 (CL555-65169, Clone:GHI/61) (red) or unstained. Cells were not fixed. Monocytes and granulocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 555 Anti-Human CD163 (GHI/61) (CL555-65169)

    FC experiment of human PBMCs using CL555-65169

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD163 (CL555-65169, Clone:GHI/61) or unstained. Cells were not fixed. Monocytes and granulocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-65169 targets CD163 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Glycoprotein preparation from hairy cell leukemia spleen 相同性解析による交差性が予測される生物種
Full Name CD163 molecule
Calculated molecular weight 1156 aa, 125 kDa
GenBank accession numberBC051281
Gene symbol CD163
Gene ID (NCBI) 9332
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

Protocols

Product Specific Protocols
FC protocol for CL Plus 555 CD163 antibody CL555-65169Download protocol
Standard Protocols
Click here to view our Standard Protocols