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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FITC Plus Anti-Human CD163 (GHI/61) Mouse IgG1 Recombinant Antibody

CD163 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

GHI/61

Cat no : FITC-65522

Synonyms

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD163 (GHI/61) Mouse Recombin (FITC-65522)

    FC experiment of human PBMCs using FITC-65522

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD163 Mouse Recombinant Antibody (FITC-65522, Clone: GHI/61) or FITC Plus Mouse IgG1 Isotype Control (MOPC-21) (FITC-65124, Clone: MOPC-21), and 5 ul APC Anti-Human CD11b (ICRF44) (APC-65116, Clone: ICRF44). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD163 (GHI/61) Mouse Recombin (FITC-65522)

    FC experiment of human PBMCs using FITC-65522

    1x10^6 FC receptor block human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD163 Mouse Recombinant Antibody (FITC-65522, Clone:GHI/61), and 5 ul APC Anti-Human CD11b (ICRF44) (APC-65116, Clone: ICRF44), and 5 ul FITC Plus Anti-Human CD163 (GHI/61) (FITC-65169, Clone: GHI/61), and FITC Plus Mouse IgG1 Isotype Control (MOPC-21) (FITC-65124, Clone: MOPC-21). Cells were not fixed.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65522 targets CD163 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Recombinant
Type Antibody
Immunogen Glycoprotein preparation from hairy cell leukemia spleen 相同性解析による交差性が予測される生物種
Full Name CD163 molecule
Calculated molecular weight 1156 aa, 125 kDa
GenBank accession numberBC051281
Gene symbol CD163
Gene ID (NCBI) 9332
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

Protocols

Product Specific Protocols
FC protocol for FITC Plus CD163 antibody FITC-65522Download protocol
Standard Protocols
Click here to view our Standard Protocols