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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD18 (TS1/18) Mouse IgG2a Recombinant Antibody

CD18 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

TS1/18

Cat no : 65595-1-MR

Synonyms

ITGB2, LCAMB, LAD, Integrin beta-2, Integrin beta 2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD18 (TS1/18) Mouse IgG2a Recombinant A (65595-1-MR)

    FC experiment of human PBMCs using 65595-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD18 Mouse IgG2a Recombinant Antibody (65595-1-MR, Clone: TS1/18) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD45. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD18 (TS1/18) Mouse IgG2a Recombinant A (65595-1-MR)

    FC experiment of human PBMCs using 65595-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD18 Mouse IgG2a Recombinant Antibody (65595-1-MR, Clone: TS1/18) (red) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65595-1-MR targets CD18 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human Beta 2 Integrin 相同性解析による交差性が予測される生物種
Full Name integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Calculated molecular weight 85 kDa
GenBank accession numberBC005861
Gene symbol CD18
Gene ID (NCBI) 3689
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

Protocols

Product Specific Protocols
FC protocol for CD18 antibody 65595-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols