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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FITC Plus Anti-Human CD18 (TS1/18) Mouse IgG2a Recombinant Antibody

CD18 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

TS1/18

Cat no : FITC-65595

Synonyms

ITGB2, LCAMB, LAD, Integrin beta-2, Integrin beta 2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD18 (TS1/18) Mouse IgG2a Rec (FITC-65595)

    FC experiment of human PBMCs using FITC-65595

    1x10^6 human PBMCs were surface stained with APC Anti-Human CD45 (HI30) (APC-65109, Clone: HI30) and 5 ul FITC Plus Anti-Human CD18 (TS1/18) Mouse IgG2a Recombinant Antibody (FITC-65595, Clone:TS1/18), or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD18 (TS1/18) Mouse IgG2a Rec (FITC-65595)

    FC experiment of human PBMCs using FITC-65595

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD18 (TS1/18) Mouse IgG2a Recombinant Antibody (FITC-65595, Clone:TS1/18)(red), or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4)(blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65595 targets CD18 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human Beta 2 Integrin 相同性解析による交差性が予測される生物種
Full Name integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Calculated molecular weight 85 kDa
GenBank accession numberBC005861
Gene symbol CD18
Gene ID (NCBI) 3689
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

Protocols

Product Specific Protocols
FC protocol for FITC Plus CD18 antibody FITC-65595Download protocol
Standard Protocols
Click here to view our Standard Protocols