FITC Anti-Mouse CD18 (M18/2)
CD18 Monoclonal Antibody for FC
Host / Isotype
Rat / IgG2a, kappa
Reactivity
Mouse
Applications
FC
Conjugate
FITC Fluorescent Dye
CloneNo.
M18/2
Cat no : FITC-65178
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
FITC-65178 targets CD18 in FC applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Mouse CTL glycoprotein 相同性解析による交差性が予測される生物種 |
| Full Name | integrin beta 2 |
| GenBank accession number | BC145644 |
| Gene symbol | CD18 |
| Gene ID (NCBI) | 16414 |
| RRID | AB_2883816 |
| Conjugate | FITC Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 494 nm / 520 nm |
| Form | Liquid |
| Purification Method | The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| Storage Buffer | PBS with 0.1% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC CD18 antibody FITC-65178 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
