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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody

CD19 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

4G7

Cat no : 65562-1-MR

Synonyms

CD19 molecule, B-lymphocyte surface antigen B4, B-lymphocyte antigen CD19, B4, B lymphocyte antigen CD19

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Anti (65562-1-MR)

    FC experiment of human PBMCs using 65562-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD19 Mouse IgG2a Recombinant Antibody (65562-1-MR, Clone: 4G7) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Anti (65562-1-MR)

    FC experiment of human PBMCs using 65562-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD19 Mouse IgG2a Recombinant Antibody (65562-1-MR, Clone: 4G7) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65562-1-MR targets CD19 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human chronic lymphocytic leukemia (CLL) cells 相同性解析による交差性が予測される生物種
Full Name CD19 molecule
Calculated molecular weight 556 aa, 61 kDa
GenBank accession numberBC006338
Gene symbol CD19
Gene ID (NCBI) 930
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

Protocols

Product Specific Protocols
FC protocol for CD19 antibody 65562-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols