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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 750 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody

CD19 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

4G7

Cat no : CL750-65562

Synonyms

CD19 molecule, B-lymphocyte surface antigen B4, B-lymphocyte antigen CD19, B4, B lymphocyte antigen CD19

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 750 Anti-Human CD19 (4G7) Mouse IgG (CL750-65562)

    FC experiment of human PBMCs using CL750-65562

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3 and 5 ul CoraLite® Plus 750 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL750-65562, Clone: 4G7) (right), or CoraLite® Plus 750 Mouse IgG2a Isotype Control (C1.18.4) (CL750-65208, Clone: C1.18.4) (left). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 750 Anti-Human CD19 (4G7) Mouse IgG (CL750-65562)

    FC experiment of human PBMCs using CL750-65562

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL750-65562, Clone: 4G7) (red), or CoraLite® Plus 750 Mouse IgG2a Isotype Control (C1.18.4) (CL750-65208, Clone: C1.18.4) (blue). Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-65562 targets CD19 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human chronic lymphocytic leukemia (CLL) cells 相同性解析による交差性が予測される生物種
Full Name CD19 molecule
Calculated molecular weight 556 aa, 61 kDa
GenBank accession numberBC006338
Gene symbol CD19
Gene ID (NCBI) 930
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

Protocols

Product Specific Protocols
FC protocol for CL Plus 750 CD19 antibody CL750-65562Download protocol
Standard Protocols
Click here to view our Standard Protocols