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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Atlantic Blue™ Anti-Human CD19 (4G7) (AB-65197)

    FC experiment of human PBMCs using AB-65197

    1X10^6 human PBMCs were surface co-stained with PE Anti-Human CD3 and 5 ul Atlantic Blue™ Anti-Human CD19 (AB-65197, Clone: 4G7) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Atlantic Blue™ Anti-Human CD19 (4G7) (AB-65197)

    FC experiment of human PBMCs using AB-65197

    1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD19 (AB-65197, Clone:4G7) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Atlantic Blue™ Anti-Human CD19 (4G7)

CD19 Monoclonal Antibody for FC
Cat No. AB-65197
Clone No.4G7

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Synonyms

B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Atlantic Blue™
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

AB-65197 targets CD19 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human chronic lymphocytic leukemia (CLL) cells 相同性解析による交差性が予測される生物種
Full Name CD19 molecule
Calculated molecular weight 556 aa, 61 kDa
GenBank accession numberBC006338
Gene Symbol CD19
Gene ID (NCBI) 930
ENSEMBL Gene IDENSG00000177455
RRIDAB_2935192
Conjugate Atlantic Blue™ Fluorescent Dye
Excitation/Emission maxima wavelengths404 nm / 458 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP15391
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

Protocols

Product Specific Protocols
FC protocol for Atlantic Blue™ CD19 antibody AB-65197Download protocol
Standard Protocols
Click here to view our Standard Protocols