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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD1a (HI149)

CD1a Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

HI149

Cat no : 65172-1-Ig

Synonyms

hTa1 thymocyte antigen, HTA1, HI149, FCB6, CD1a molecule

Validation Data Gallery

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  • Flow cytometry (FC) experiment of MOLT-4 cells using Anti-Human CD1a (HI149) (65172-1-Ig)

    FC experiment of MOLT-4 using 65172-1-Ig

    0.5x10^6 MOLT-4 cells were surface stained with 0.2 ug Anti-Human CD1a (65172-1-Ig, Clone: HI149) (red) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) (blue), and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Cells were not fixed.

  • Flow cytometry (FC) experiment of MOLT-4 cells using Anti-Human CD1a (HI149) (65172-1-Ig)

    FC experiment of MOLT-4 using 65172-1-Ig

    0.5x10^6 MOLT-4 cells were surface stained with 0.2 ug Anti-Human CD1a (65172-1-Ig, Clone: HI149) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21), and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Cells were not fixed.

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Tested Applications

Positive FC detected inMOLT-4 cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65172-1-Ig targets CD1a in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human thymocytes 相同性解析による交差性が予測される生物種
Full Name CD1a molecule
Calculated molecular weight 327 aa, 37 kDa
GenBank accession numberBC031645
Gene symbol CD1A
Gene ID (NCBI) 909
RRIDAB_2918448
Conjugate Unconjugated
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD1a is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. During protein synthesis and maturation, CD1 family members bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface.

Protocols

Product Specific Protocols
FC protocol for CD1a antibody 65172-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols