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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APC Anti-Human CD1a (HI149)

CD1a Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

HI149

Cat no : APC-65172

Synonyms

hTa1 thymocyte antigen, HTA1, HI149, FCB6, CD1a molecule

Validation Data Gallery

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  • Flow cytometry (FC) experiment of MOLT-4 cells using APC Anti-Human CD1a (HI149) (APC-65172)

    FC experiment of MOLT-4 using APC-65172

    1X10^6 MOLT-4 cells were surface stained with 10 ul APC Anti-Human CD1a (APC-65172, Clone:HI149), or Mouse IgG1 Isotype Control. Cells were not fixed.

  • Flow cytometry (FC) experiment of MOLT-4 cells using APC Anti-Human CD1a (HI149) (APC-65172)

    FC experiment of MOLT-4 using APC-65172

    1X10^6 MOLT-4 cells were surface stained with 10 ul APC Anti-Human CD1a (APC-65172, Clone:HI149), or Mouse IgG1 Isotype Control. Cells were not fixed.

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Tested Applications

Positive FC detected inMOLT-4 cells

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65172 targets CD1a in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human thymocytes 相同性解析による交差性が予測される生物種
Full Name CD1a molecule
Calculated molecular weight 327 aa, 37 kDa
GenBank accession numberBC031645
Gene symbol CD1A
Gene ID (NCBI) 909
RRIDAB_2883040
Conjugate APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Form Liquid
Purification MethodThe purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD1a is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. During protein synthesis and maturation, CD1 family members bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface.

Protocols

Product Specific Protocols
FC protocol for APC CD1a antibody APC-65172Download protocol
Standard Protocols
Click here to view our Standard Protocols