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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated CD1d Monoclonal anti (CL488-66257)

    WB analysis using CL488-66257

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66257 (CD1d antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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CoraLite® Plus 488-conjugated CD1d Monoclonal antibody

CD1d Monoclonal Antibody for WB
Cat No. CL488-66257
Clone No.1C12F1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB

Synonyms

CD1A, CD1D, CD1d molecule, R3, R3G1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMOLT-4 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66257 targets CD1d in WB applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD1d fusion protein Ag11108 相同性解析による交差性が予測される生物種
Full Name CD1d molecule
Calculated molecular weight 335 aa, 38 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC027926
Gene Symbol CD1D
Gene ID (NCBI) 912
RRIDAB_3084226
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP15813
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD1d is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on NKT cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines. The molecular weight of unglycosylated CD1d is 38 kDa, while glycosylated form of CD1d is 48-55 kDa.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 CD1d antibody CL488-66257Download protocol
Standard Protocols
Click here to view our Standard Protocols