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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant An (65565-1-MR)

    FC experiment of human PBMCs using 65565-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Mouse Recombinant Antibody (65565-1-MR, Clone:TS1/8) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant An (65565-1-MR)

    FC experiment of human PBMCs using 65565-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Mouse Recombinant Antibody (65565-1-MR, Clone: TS1/8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (right), or 0.25 ug Anti-Human CD2 (65192-1-Ig, Clone: TS1/8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (left). Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

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Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody

CD2 Uni-rAbTM Recombinant Antibody for FC
Cat No. 65565-1-MR
Clone No.TS1/8

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

CD2, LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65565-1-MR targets CD2 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD2 molecule
Calculated molecular weight 351 aa, 39 kDa
GenBank accession numberBC033583
Gene Symbol CD2
Gene ID (NCBI) 914
ENSEMBL Gene IDENSG00000116824
RRIDAB_3670319
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide , pH 7.3
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Protocols

Product Specific Protocols
FC protocol for CD2 antibody 65565-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols