Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only

Name: Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only
Brand: Proteintech
SKU: 65565-1-PBS

CD2 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

Conjugate

Unconjugated

CloneNo.

TS1/8

Review Product

Cat no : 65565-1-PBS

Synonyms

LFA-3 receptor, LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor

Validation Data Gallery

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AllFC

FC experiment of human PBMCs using 65565-1-MR (same clone as 65565-1-PBS)
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Mouse Recombinant Antibody (65565-1-MR, Clone:TS1/8) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65565-1-PBS in a different storage buffer formulation.
×
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Mouse Recombinant Antibody (65565-1-MR, Clone:TS1/8) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65565-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65565-1-PBS
1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only (65565-1-PBS, Clone:TS1/8) , or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were then stained with FITC Plus Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.
×
1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only (65565-1-PBS, Clone:TS1/8) , or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were then stained with FITC Plus Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65565-1-PBS
1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only (65565-1-PBS, Clone:TS1/8) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8)(right), or 0.125 ug Anti-Human CD2 (TS1/8) (65192-1-Ig, Clone: TS1/8) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8)(left). Cells were then stained with FITC Plus Anti-Human CD19 (HIB19) . Cells were not fixed. Lymphocytes were gated.
×
1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only (65565-1-PBS, Clone:TS1/8) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8)(right), or 0.125 ug Anti-Human CD2 (TS1/8) (65192-1-Ig, Clone: TS1/8) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8)(left). Cells were then stained with FITC Plus Anti-Human CD19 (HIB19) . Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65565-1-MR (same clone as 65565-1-PBS)
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Mouse Recombinant Antibody (65565-1-MR, Clone: TS1/8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (right), or 0.25 ug Anti-Human CD2 (65192-1-Ig, Clone: TS1/8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (left). Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65565-1-PBS in a different storage buffer formulation.
×
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Mouse Recombinant Antibody (65565-1-MR, Clone: TS1/8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (right), or 0.25 ug Anti-Human CD2 (65192-1-Ig, Clone: TS1/8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (left). Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65565-1-PBS in a different storage buffer formulation.

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Cat No. 65565-1-PBS

Size/Concentration

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Tested Applications

Positive FC detected in

human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65565-1-PBS targets CD2 in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2a
Class	Recombinant
Type	Antibody
Immunogen	N/A 相同性解析による交差性が予測される生物種
Full Name	CD2 molecule
Calculated molecular weight	351 aa, 39 kDa
GenBank accession number	BC033583
Gene symbol	CD2
Gene ID (NCBI)	914
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C. Stable for one year after shipment.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Protocols

Product Specific Protocols
FC protocol for CD2 antibody 65565-1-PBS	Download protocol

Standard Protocols
Click here to view our Standard Protocols

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カスタム対応

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