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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only

CD2 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

TS1/8

Cat no : 65565-1-PBS

Synonyms

LFA-3 receptor, LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant An (65565-1-MR)

    FC experiment of human PBMCs using 65565-1-MR (same clone as 65565-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Mouse Recombinant Antibody (65565-1-MR, Clone:TS1/8) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65565-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Ant (65565-1-PBS)

    FC experiment of human PBMCs using 65565-1-PBS

    1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only (65565-1-PBS, Clone:TS1/8) , or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were then stained with FITC Plus Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Ant (65565-1-PBS)

    FC experiment of human PBMCs using 65565-1-PBS

    1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant Antibody, PBS Only (65565-1-PBS, Clone:TS1/8) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8)(right), or 0.125 ug Anti-Human CD2 (TS1/8) (65192-1-Ig, Clone: TS1/8) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8)(left). Cells were then stained with FITC Plus Anti-Human CD19 (HIB19) . Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD2 (TS1/8) Mouse IgG2a Recombinant An (65565-1-MR)

    FC experiment of human PBMCs using 65565-1-MR (same clone as 65565-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Mouse Recombinant Antibody (65565-1-MR, Clone: TS1/8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (right), or 0.25 ug Anti-Human CD2 (65192-1-Ig, Clone: TS1/8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (left). Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65565-1-PBS in a different storage buffer formulation.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65565-1-PBS targets CD2 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD2 molecule
Calculated molecular weight 351 aa, 39 kDa
GenBank accession numberBC033583
Gene symbol CD2
Gene ID (NCBI) 914
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Protocols

Product Specific Protocols
FC protocol for CD2 antibody 65565-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols