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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD2 Recombinant antibody (84108-5-RR)

    WB analysis using 84108-5-RR (same clone as 84108-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84108-5-RR (CD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84108-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD2 Recombinant antibody (84108-5-RR)

    WB analysis using 84108-5-RR (same clone as 84108-5-PBS)

    NK-92 cells were subjected to SDS PAGE followed by western blot with 84108-5-RR (CD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84108-5-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01045-4

    Sandwich ELISA standard curve of MP01045-4

    Sandwich ELISA standard curve of MP01045-4, Human CD2 Recombinant Matched Antibody Pair - PBS only. 84108-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1271. 84108-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

  • Sample test of MP01045-4

    Sample test of MP01045-4

    Jurkat and Molt-4 cell lysate were measured. The human CD2 concentration of detected samples was determined to be 8,318.1 pg/mL (based on a 1.0 mg/mL extract load) in Jurkat cell lysate and 679.3 pg/mL (based on a 4.5 mg/mL extract load) in Molt-4 cell lysate.

  • Sample test of MP01045-4

    Sample test of MP01045-4

    Serum of six individual healthy human donors was measured. The human CD2 concentration of detected samples was determined to be 167.0 pg/mL with a range of 119.9 - 223.9 pg/mL.

  • Affinity and Kinetic Characterization of 84108-5-RR

    Affinity and Kinetic Characterization of 84108-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84108-5-RR against Human CD2 were performed. The affinity constant is 0.494 nM.

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CD2 Recombinant antibody, PBS Only (Capture)

CD2 Uni-rAbTM Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84108-5-PBS
Clone No.241222B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor, CD2 molecule

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84108-5-PBS targets CD2 as part of a matched antibody pair:

MP01045-4: 84108-5-PBS capture and 84108-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD2 molecule
Calculated molecular weight 351 aa, 39 kDa
Observed molecular weight50 kDa
GenBank accession numberBC033583
Gene Symbol CD2
Gene ID (NCBI) 914
ENSEMBL Gene IDENSG00000116824
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06729
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.