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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD2 Recombinant antibody (84108-5-RR)

    WB analysis using 84108-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84108-5-RR (CD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CD2 Recombinant antibody (84108-5-RR)

    WB analysis using 84108-5-RR

    NK-92 cells were subjected to SDS PAGE followed by western blot with 84108-5-RR (CD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD2 Recombinant antibody (84108-5-RR)

    IF Staining of Jurkat using 84108-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells (positive cell) and HeLa cell (negative cell) using CD2 antibody (84108-5-RR, Clone: 241222B8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84108-5-RR

    Affinity and Kinetic Characterization of 84108-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84108-5-RR against Human CD2 were performed. The affinity constant is 0.494 nM.

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CD2 Recombinant antibody

CD2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84108-5-RR
Clone No.241222B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

241222B8, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, NK-92 cells
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84108-5-RR targets CD2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CD2 fusion protein Eg1271 相同性解析による交差性が予測される生物種
Full Name CD2 molecule
Calculated molecular weight 351 aa, 39 kDa
Observed molecular weight50 kDa
GenBank accession numberBC033583
Gene Symbol CD2
Gene ID (NCBI) 914
ENSEMBL Gene IDENSG00000116824
RRIDAB_3671675
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06729
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Protocols

Product Specific Protocols
WB protocol for CD2 antibody 84108-5-RRDownload protocol
IF protocol for CD2 antibody 84108-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols