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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 555 Anti-Human CD2 (TS1/8)

CD2 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

TS1/8

Cat no : CL555-65192

Synonyms

CD2, CD2 molecule, Erythrocyte receptor, FLJ46032, LFA 2, LFA 3 receptor, Rosette receptor, SRBC, T cell surface antigen CD2, T11

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 555 Anti-Human CD2 (TS1/8) (CL555-65192)

    FC experiment of human PBMCs using CL555-65192

    1X10^6 human PBMCs were surface co-stained with APC Anti-Human CD3 and 5 ul CoraLite® Plus 555 Anti-Human CD2 (CL555-65192, Clone:TS1/8) or Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 555 Anti-Human CD2 (TS1/8) (CL555-65192)

    FC experiment of human PBMCs using CL555-65192

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD2 (CL555-65192, Clone:TS1/8) (red) or Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-65192 targets CD2 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD2 molecule
Calculated molecular weight 351 aa, 39 kDa
GenBank accession numberBC033583
Gene symbol CD2
Gene ID (NCBI) 914
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Protocols

Product Specific Protocols
FC protocol for CL Plus 555 CD2 antibody CL555-65192Download protocol
Standard Protocols
Click here to view our Standard Protocols