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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD20 (2H7) Mouse IgG2a Recombinant Antibody

CD20 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

2H7

Cat no : 65575-1-MR

Synonyms

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD20 (2H7) Mouse IgG2a Recombinant Anti (65575-1-MR)

    FC experiment of human PBMCs using 65575-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD20 Mouse IgG2a Recombinant Antibody (65575-1-MR, Clone: 2H7) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD20 (2H7) Mouse IgG2a Recombinant Anti (65575-1-MR)

    FC experiment of human PBMCs using 65575-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD20 Mouse IgG2a Recombinant Antibody (65575-1-MR, Clone: 2H7) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD20 (2H7) Mouse IgG2a Recombinant Anti (65575-1-MR)

    FC experiment of human PBMCs using 65575-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD20 Mouse IgG2a Recombinant Antibody (65575-1-MR, Clone:2H7)(left) or 0.25 ug Anti-Human CD20 (65085-1-Ig, Clone: 2H7)(right) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were not fixed. lymphocyte were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65575-1-MR targets CD20 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human tonsillar B cells 相同性解析による交差性が予測される生物種
Full Name membrane-spanning 4-domains, subfamily A, member 1
Calculated molecular weight 297 aa, 33 kDa
GenBank accession numberBC002807
Gene symbol CD20
Gene ID (NCBI) 931
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).

Protocols

Product Specific Protocols
FC protocol for CD20 antibody 65575-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols