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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD20 (2H7) Mouse IgG2a Recombinant Antibody, PBS Only

CD20 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

2H7

Cat no : 65575-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD20 (2H7) Mouse IgG2a Recombinant Anti (65575-1-MR)

    FC experiment of human PBMCs using 65575-1-MR (same clone as 65575-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD20 Mouse IgG2a Recombinant Antibody (65575-1-MR, Clone: 2H7) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65575-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD20 (2H7) Mouse IgG2a Recombinant Anti (65575-1-MR)

    FC experiment of human PBMCs using 65575-1-MR (same clone as 65575-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD20 Mouse IgG2a Recombinant Antibody (65575-1-MR, Clone: 2H7) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65575-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD20 (2H7) Mouse IgG2a Recombinant Anti (65575-1-MR)

    FC experiment of human PBMCs using 65575-1-MR (same clone as 65575-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD20 Mouse IgG2a Recombinant Antibody (65575-1-MR, Clone:2H7)(left) or 0.25 ug Anti-Human CD20 (65085-1-Ig, Clone: 2H7)(right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. lymphocyte were gated. This data was developed using the same antibody clone with 65575-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65575-1-PBS targets CD20 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human tonsillar B cells 相同性解析による交差性が予測される生物種
Full Name membrane-spanning 4-domains, subfamily A, member 1
Calculated molecular weight 297 aa, 33 kDa
GenBank accession numberBC002807
Gene symbol CD20
Gene ID (NCBI) 931
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).