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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cardinal Red™ Anti-Human CD20 (2H7)

CD20 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human

Applications

FC

Conjugate

Cardinal Red™ Fluorescent Dye

CloneNo.

2H7

Cat no : CR-65085

Synonyms

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Cardinal Red™ Anti-Human CD20 (2H7) (CR-65085)

    FC experiment of human PBMCs using CR-65085

    1X10^6 human PBMCs were surface co-stained with APC Anti-Human CD19 and 5 ul Cardinal Red™ Anti-Human CD20 (CR-65085, Clone:2H7) or Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Cardinal Red™ Anti-Human CD20 (2H7) (CR-65085)

    FC experiment of human PBMCs using CR-65085

    1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD20 (CR-65085, Clone:2H7) or Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CR-65085 targets CD20 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Human tonsillar B cells 相同性解析による交差性が予測される生物種
Full Name membrane-spanning 4-domains, subfamily A, member 1
Calculated molecular weight 297 aa, 33 kDa
GenBank accession numberBC002807
Gene symbol CD20
Gene ID (NCBI) 931
Conjugate Cardinal Red™ Fluorescent Dye
Excitation/Emission maxima wavelengths592 nm / 611 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).

Protocols

Product Specific Protocols
FC protocol for Cardinal Red™ CD20 antibody CR-65085Download protocol
Standard Protocols
Click here to view our Standard Protocols