CoraLite® Plus 488-conjugated CD206 Polyclonal antibody
CD206 Polyclonal Antibody for FC (Intra), IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-18704
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | mouse lung tissue |
| Positive FC detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-18704 targets CD206 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | mannose receptor, C type 1 |
| Calculated molecular weight | 166 kDa |
| Observed molecular weight | 165-180 kDa |
| GenBank accession number | NM_002438 |
| Gene symbol | MRC1 |
| Gene ID (NCBI) | 4360 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Background
CD206 (macrophage mannose receptor 1) is a lectin-type endocytic receptor expressed on selected macrophages, dendritic cells, and non-vascular endothelium and plays a role in antigen processing and presentation, phagocytosis, and intracellular signaling.
1. What is the molecular weight of CD206?
The molecular size of full-length CD206 is 170-180 kDa, depending on the exact tissue-specific glycosylation pattern (PMID: 19427834). Additionally, CD206 can be cleaved off and a soluble form (sMR) lacking the tail, with a slightly lower molecular weight, can be released to the cell medium (PMID: 9722572).
2. What is the subcellular localization of CD206?
CD206 is a type I membrane protein composed of a large extracellular multidomain, a transmembrane domain, and a short cytoplasmic tail. It is present at the plasma membrane and in endosomes, as CD206 undergoes constant recycling between the plasma membrane and endosomal compartment.
3. Is CD206 post-translationally modified?
CD206 undergoes quite extensive post-translational modifications, predominantly N-linked glycosylation that affects ligand binding recognition and affinity (PMID: 22966131).
4. Can CD206 marker be used as a marker of M2 macrophages?
The activation of macrophages with various stimuli leads to their polarization into classical (M1) or alternatively activated (M2) subtypes spectrums and both subtypes differ in their regulatory and effector functions (PMID: 24669294). Pathogens and IFN-γ promote M1 polarization, while IL-4 released during parasite infections and allergen response promotes M2 polarization. Classically, the markers of M2 macrophages include CD206, as well as arginase-1 (ARG1; https://www.ptglab.com/products/ARG1-Antibody-16001-1-AP.htm), CD163 (https://www.ptglab.com/products/CD163-Antibody-16646-1-AP.htm), and thrombospondin 1 (TSP1/ THBS1; https://www.ptglab.com/products/TSP1-Antibody-18304-1-AP.htm).
5. How can you polarize macrophages into M2 direction?
One of the most commonly used methods is stimulation by the addition of IL-4 cytokine. We recommend using our animal-free human IL-4 (https://www.ptglab.com/products/recombinant-human-il-4.htm).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CD206 antibody CL488-18704 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
