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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD21/CD35 Recombinant antibody (84565-2-RR)

    WB analysis using 84565-2-RR (same clone as 84565-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84565-2-RR (CD21 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84565-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD21/CD35 Recombinant antibody (84565-2-RR)

    WB analysis using 84565-2-RR (same clone as 84565-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84565-2-RR (CD21/CD35 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84565-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD21/CD35 Recombinant antibody (84565-2-RR)

    IHC staining of mouse spleen using 84565-2-RR (same clone as 84565-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84565-2-RR (CD21 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84565-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD21/CD35 Recombinant antibody (84565-2-RR)

    IHC staining of mouse spleen using 84565-2-RR (same clone as 84565-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84565-2-RR (CD21 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84565-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01407-1

    Cytometric bead array standard curve of MP01407-1

    Cytometric bead array standard curve of MP01407-1, MOUSE CD21 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84565-2-PBS. Detection antibody: 84565-3-PBS. Standard: Eg1396. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84565-2-RR

    Affinity and Kinetic Characterization of 84565-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84565-2-RR against Mouse CD21/CD35 were performed. The affinity constant is 4.84 nM.

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Mouse CD21/CD35 Recombinant antibody, PBS Only (Capture)

CD21/CD35 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 84565-2-PBS
Clone No.241957E6

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

CD21, Cr2, 241957E6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84565-2-PBS targets CD21/CD35 as part of a matched antibody pair:

MP01407-1: 84565-2-PBS capture and 84565-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name complement receptor 2
Calculated molecular weight113kDa
Observed molecular weight160-210 kDa
GenBank accession numberAAA37448.1
Gene Symbol Cr2
Gene ID (NCBI) 12902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19070
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 short consensus repeats (SCRs) (PMID: 6230668; 2551147). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, binds C3b and C4b. The murine Cr2 gene encodes for both CD21 and CD35 (PMID: 2139460; 19559484). Mouse CD35 comprises 21 SCRs while mouse CD21 is identical to mouse CD35 except that it lacks the first 6 SCRs. CD21/CD35 forms trimolecular complexes with CD19 and CD81 and plays roles in the regulation of humoral immune responses (PMID: 11043778).