Validation Data Gallery
Tested Applications
| Positive WB detected in | A20 cells, mouse spleen tissue, rat spleen tissue |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84565-2-RR targets CD21/CD35 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | complement receptor 2 |
| Calculated molecular weight | 113kDa |
| Observed molecular weight | 160-210 kDa |
| GenBank accession number | AAA37448.1 |
| Gene Symbol | Cr2 |
| Gene ID (NCBI) | 12902 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P19070 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 short consensus repeats (SCRs) (PMID: 6230668; 2551147). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, binds C3b and C4b. The murine Cr2 gene encodes for both CD21 and CD35 (PMID: 2139460; 19559484). Mouse CD35 comprises 21 SCRs while mouse CD21 is identical to mouse CD35 except that it lacks the first 6 SCRs. CD21/CD35 forms trimolecular complexes with CD19 and CD81 and plays roles in the regulation of humoral immune responses (PMID: 11043778).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD21/CD35 antibody 84565-2-RR | Download protocol |
| IHC protocol for CD21/CD35 antibody 84565-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |