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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD22 Recombinant antibody (84265-2-RR)

    WB analysis using 84265-2-RR (same clone as 84265-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84265-2-RR (CD22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84265-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01173-3

    Sandwich ELISA standard curve of MP01173-3

    Sandwich ELISA standard curve of MP01173-3, Human CD22 Recombinant Matched Antibody Pair - PBS only. 84265-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02324. 84265-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01173-3

    Sample test of MP01173-3

    The mean CD22 concentration was determined to be 445.39 ng/mL in Raji cell extract based on a 3.00 mg/mL extract load and 185.59 ng/mL in Ramos cell extract based on a 1.40 mg/mL extract load.

  • Sample test of MP01173-3

    Sample test of MP01173-3

    Serum of six individual healthy human donors was measured. The CD22 concentration of detected samples was determined to be 0.36 ng/mL with a range of 0.25-0.54 ng/mL.

  • Cytometric bead array standard curve of MP01173-1

    Cytometric bead array standard curve of MP01173-1

    Cytometric bead array standard curve of MP01173-1, CD22 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84265-2-PBS. Detection antibody: 84265-3-PBS. Standard: RP02324. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01173-3

    Cytometric bead array standard curve of MP01173-3

    Cytometric bead array standard curve of MP01173-3, CD22 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84265-4-PBS. Detection antibody: 84265-2-PBS. Standard: RP02324. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84265-2-RR

    Affinity and Kinetic Characterization of 84265-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84265-2-RR against Human CD22 were performed. The affinity constant is 61.3 pM.

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CD22 Recombinant antibody, PBS Only (Capture/Detector)

CD22 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84265-2-PBS
Clone No.241314G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1 mg/ml
100 μg, 1 mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84265-2-PBS targets CD22 as part of a matched antibody pair:

MP01173-1: 84265-2-PBS capture and 84265-3-PBS detection (validated in Cytometric bead array)

MP01173-3: 84265-4-PBS capture and 84265-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name CD22 molecule
Calculated molecular weight 847 aa, 95 kDa
Observed molecular weight130-140 kDa
GenBank accession numberBC109306
Gene Symbol CD22
Gene ID (NCBI) 933
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20273
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.