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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD22 Recombinant antibody

CD22 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241314G10

Cat no : 84265-2-RR

Synonyms

CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD22 Recombinant antibody (84265-2-RR)

    WB analysis using 84265-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84265-2-RR (CD22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84265-2-RR

    Affinity and Kinetic Characterization of 84265-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84265-2-RR against Human CD22 were performed. The affinity constant is 61.3 pM.

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Tested Applications

Positive WB detected inDaudi cells, Raji cells, Ramos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84265-2-RR targets CD22 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name CD22 molecule
Calculated molecular weight 847 aa, 95 kDa
Observed molecular weight130-140 kDa
GenBank accession numberBC109306
Gene symbol CD22
Gene ID (NCBI) 933
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.

Protocols

Product Specific Protocols
WB protocol for CD22 antibody 84265-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols