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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®488-conjugated CD22 Monoclonal antibody

CD22 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF-P

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

7A2F1

Cat no : CL488-66103

Synonyms

B cell receptor CD22, BL CAM, CD22, CD22 molecule, SIGLEC 2, SIGLEC2, T cell surface antigen Leu 14

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®488-conjugated CD22 Monoclonal antibody (CL488-66103)

    IF Staining of human tonsillitis using CL488-66103

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66103 (CD22 antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®488-conjugated CD22 Monoclonal antibody (CL488-66103)

    IF Staining of human tonsillitis using CL488-66103

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66103 (CD22 antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CoraLite®488-conjugated CD22 Monoclonal antibody (CL488-66103)

    IF Staining of human appendicitis using CL488-66103

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using CoraLite®488 CD22 antibody (CL488-66103, Clone: 7A2F1 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-66103 is CD22 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD22 fusion protein Ag17986 相同性解析による交差性が予測される生物種
Full Name CD22 molecule
Calculated molecular weight 847 aa, 95 kDa
GenBank accession numberBC109306
Gene symbol CD22
Gene ID (NCBI) 933
RRIDAB_2883259
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths491 nm / 516 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell. And this antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
IF protocol for CL488 CD22 antibody CL488-66103Download protocol
Standard Protocols
Click here to view our Standard Protocols