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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PE Anti-Human CD235a (HIR2)

CD235a Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

HIR2

Cat no : PE-65174

Synonyms

CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human red blood cells using PE Anti-Human CD235a (HIR2) (PE-65174)

    FC experiment of human red blood cells using PE-65174

    10 ul human whole blood was surface stained with 5 ul PE Anti-Human CD235a (HIR2) (PE-65174) or PE Mouse IgG2b Isotype Control. Cells were not fixed. Red blood cells were gated.

  • Flow cytometry (FC) experiment of human red blood cells using PE Anti-Human CD235a (HIR2) (PE-65174)

    FC experiment of human red blood cells using PE-65174

    10 ul human whole blood was surface stained with 5 ul PE Anti-Human CD235a (HIR2) (PE-65174) or PE Mouse IgG2b Isotype Control. Cells were not fixed. Red blood cells were gated.

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Tested Applications

Positive FC detected inhuman red blood cells

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65174 targets CD235a in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Synthetic peptide (Human, N-terminal) 相同性解析による交差性が予測される生物種
Full Name glycophorin A (MNS blood group)
Calculated molecular weight 150 aa, 16 kDa
GenBank accession numberBC005319
Gene symbol Glycophorin A
Gene ID (NCBI) 2993
RRIDAB_2883948
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodThe purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

Protocols

Product Specific Protocols
FC protocol for PE CD235a antibody PE-65174Download protocol
Standard Protocols
Click here to view our Standard Protocols