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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD244 Recombinant antibody, PBS Only (Detector)

CD244 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241260C6

Cat no : 84086-1-PBS

Synonyms

SLAMF4, NKR2B4, NK cell activation-inducing ligand, NAIL, h2B4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD244 Recombinant antibody (84086-1-RR)

    WB analysis using 84086-1-RR (same clone as 84086-1-PBS)

    NK-92 cells were subjected to SDS PAGE followed by western blot with 84086-1-RR (CD244 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84086-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD244 Recombinant antibody (84086-1-RR)

    IHC staining of human tonsillitis using 84086-1-RR (same clone as 84086-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84086-1-RR (CD244 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84086-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD244 Recombinant antibody (84086-1-RR)

    IHC staining of human tonsillitis using 84086-1-RR (same clone as 84086-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84086-1-RR (CD244 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84086-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01008-3

    Cytometric bead array standard curve of MP01008-3

    Cytometric bead array standard curve of MP01008-3, CD244 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84086-2-PBS. Detection antibody: 84086-1-PBS. Standard: Eg32087. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84086-1-RR

    Affinity and Kinetic Characterization of 84086-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84086-1-RR against Human CD244 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84086-1-PBS targets CD244 as part of a matched antibody pair:

MP01008-3: 84086-2-PBS capture and 84086-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD244 molecule, natural killer cell receptor 2B4
Calculated molecular weight 365 aa, 41 kDa
GenBank accession numberBC053985
Gene symbol CD244
Gene ID (NCBI) 51744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).