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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD244 Recombinant antibody

CD244 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241260C6

Cat no : 84086-1-RR

Synonyms

SLAMF4, NKR2B4, NK cell activation-inducing ligand, NAIL, h2B4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD244 Recombinant antibody (84086-1-RR)

    WB analysis using 84086-1-RR

    NK-92 cells were subjected to SDS PAGE followed by western blot with 84086-1-RR (CD244 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD244 Recombinant antibody (84086-1-RR)

    IHC staining of human tonsillitis using 84086-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84086-1-RR (CD244 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD244 Recombinant antibody (84086-1-RR)

    IHC staining of human tonsillitis using 84086-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84086-1-RR (CD244 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84086-1-RR

    Affinity and Kinetic Characterization of 84086-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84086-1-RR against Human CD244 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inNK-92 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84086-1-RR is CD244 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD244 molecule, natural killer cell receptor 2B4
Calculated molecular weight 365 aa, 41 kDa
GenBank accession numberBC053985
Gene symbol CD244
Gene ID (NCBI) 51744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).

Protocols

Product Specific Protocols
WB protocol for CD244 antibody 84086-1-RRDownload protocol
IHC protocol for CD244 antibody 84086-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols