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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Cardinal Red™ Anti-Human CD244 (2B4.69) (CR-65270)

    FC experiment of human PBMCs using CR-65270

    1X10^6 human PBMCs were surface co-stained with CL750 Anti-Human CD56 and 5 ul Cardinal Red™ Anti-Human CD244 (CR-65270, Clone:2B4.69), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Cardinal Red™ Anti-Human CD244 (2B4.69) (CR-65270)

    FC experiment of human PBMCs using CR-65270

    1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD244 (CR-65270, Clone:2B4.69), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Cardinal Red™ Anti-Human CD244 (2B4.69)

CD244 Monoclonal Antibody for FC
Cat No. CR-65270
Clone No.2B4.69

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Synonyms

2B4, CD244, h2B4, NAIL, NKR2B4, SLAMF4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Cardinal Red™
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is N/A per 10^6 cells in a 100 µl suspension or N/A per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CR-65270 targets CD244 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD244 molecule, natural killer cell receptor 2B4
GenBank accession numberBC028073
Gene Symbol CD244
Gene ID (NCBI) 51744
RRIDAB_3084990
Conjugate Cardinal Red™ Fluorescent Dye
Excitation/Emission maxima wavelengths592 nm / 611 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).

Protocols

Product Specific Protocols
FC protocol for Cardinal Red™ CD244 antibody CR-65270Download protocol
Standard Protocols
Click here to view our Standard Protocols