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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Atlantic Blue™ Anti-Human CD25 (BC96)

CD25 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Atlantic Blue™ Fluorescent Dye

CloneNo.

BC96

Cat no : AB-65096

Synonyms

CD25, IDDM10, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA, IL2R, IL2RA, IL-2RA, interleukin 2 receptor, alpha, p55, TAC antigen, TCGFR

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Atlantic Blue™ Anti-Human CD25 (BC96) (AB-65096)

    FC experiment of human PBMCs using AB-65096

    1x10^6 PHA treated (3 days) human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul Atlantic Blue™ Anti-Human CD25 (AB-65096, Clone:BC96) (red) or Atlantic Blue™ Mouse IgG1 Isotype Control (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD25 (AB-65096, Clone:BC96) (black dashed). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Atlantic Blue™ Anti-Human CD25 (BC96) (AB-65096)

    FC experiment of human PBMCs using AB-65096

    1x10^6 PHA treated (3 days) human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul Atlantic Blue™ Anti-Human CD25 (AB-65096, Clone: BC96) (right) or Atlantic Blue™ Mouse IgG1 Isotype Control (left). 1x10^6 untreated human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD25 (AB-65096, Clone:BC96) (center). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes were gated.

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Tested Applications

Positive FC detected inPHA treated human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

AB-65096 targets CD25 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name interleukin 2 receptor, alpha
Calculated molecular weight 272 aa, 31 kDa
GenBank accession numberBC156627
Gene symbol IL2RA
Gene ID (NCBI) 3559
Conjugate Atlantic Blue™ Fluorescent Dye
Excitation/Emission maxima wavelengths404 nm / 458 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).

Protocols

Product Specific Protocols
FC protocol for Atlantic Blue™ CD25 antibody AB-65096Download protocol
Standard Protocols
Click here to view our Standard Protocols