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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488 Anti-Human CD27 (O323)

CD27 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

O323

Cat no : CL488-65132

Synonyms

CD27, CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27, T14, TNFRSF7, Tp55

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using CoraLite® Plus 488 Anti-Human CD27 (O323) (CL488-65132)

    FC experiment of human peripheral blood lymphocytes using CL488-65132

    1X10^6 human peripheral blood lymphocytes were surface stained with APC-Anti-Human CD19 (APC-65145, Clone: SJ25C1) and 5.00 ul CoraLite® Plus 488-conjugated Anti-Human CD27 (CL488-65132, Clone: O323). Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using CoraLite® Plus 488 Anti-Human CD27 (O323) (CL488-65132)

    FC experiment of human peripheral blood lymphocytes using CL488-65132

    1X10^6 human peripheral blood lymphocytes were surface stained with APC-Anti-Human CD19 (APC-65145, Clone: SJ25C1) and CoraLite® Plus 488-conjugated Mouse IgG1 isotype control antibody. Cells were not fixed.

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Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65132 targets CD27 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD27 molecule
Calculated molecular weight 29 kDa
GenBank accession numberBC012160
Gene symbol CD27
Gene ID (NCBI) 939
RRIDAB_2883194
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD27 antibody CL488-65132Download protocol
Standard Protocols
Click here to view our Standard Protocols