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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using B7-H3/CD276 Recombinant antibody (83152-5-RR)

    WB analysis using 83152-5-RR (same clone as 83152-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83152-5-RR (B7-H3/CD276 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83152-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83152-5-RR

    Affinity and Kinetic Characterization of 83152-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83152-5-RR against Human CD276 were performed. The affinity constant is 0.607 nM.

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B7-H3/CD276 Recombinant antibody, PBS Only

B7-H3/CD276 Recombinant Antibody for WB, Indirect ELISA
Cat No. 83152-5-PBS
Clone No.230305A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

CD276, B7-H3, B7H3, B7 homolog 3, B7 H3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83152-5-PBS targets B7-H3/CD276 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD276 molecule
Calculated molecular weight57 kDa
GenBank accession numberNM_001024736
Gene Symbol CD276
Gene ID (NCBI) 80381
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ5ZPR3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.