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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

B7-H3/CD276 Recombinant antibody

B7-H3/CD276 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230305A10

Cat no : 83152-5-RR

Synonyms

4Ig B7 H3, B7 H3, B7 homolog 3, B7H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule, UNQ309/PRO352

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using B7-H3/CD276 Recombinant antibody (83152-5-RR)

    WB analysis using 83152-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83152-5-RR (B7-H3/CD276 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83152-5-RR

    Affinity and Kinetic Characterization of 83152-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83152-5-RR against Human CD276 were performed. The affinity constant is 0.607 nM.

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Tested Applications

Positive WB detected inU2OS cells, HEK-293 cells, HeLa cells, LNCaP cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83152-5-RR is B7-H3/CD276 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD276 molecule
Calculated molecular weight57 kDa
Observed molecular weight95-100 kDa
GenBank accession numberNM_001024736
Gene symbol CD276
Gene ID (NCBI) 80381
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).

Protocols

Product Specific Protocols
WB protocol for B7-H3/CD276 antibody 83152-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols