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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD28 Recombinant antibody, PBS Only

CD28 Recombinant Antibody for ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

ELISA, WB

Conjugate

Unconjugated

CloneNo.

240354D9

Cat no : 83383-7-PBS

Synonyms

Tp44, T-cell-specific surface glycoprotein CD28, CD28 molecule, 240354D9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD28 Recombinant antibody (83383-7-RR)

    WB analysis using 83383-7-RR (same clone as 83383-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83383-7-RR (CD28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83383-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83383-7-RR

    Affinity and Kinetic Characterization of 83383-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83383-7-RR against Human CD28 were performed. The affinity constant is 2.75 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83383-7-PBS is CD28 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD28 molecule
Calculated molecular weight 220 aa, 25 kDa
GenBank accession numberBC093698
Gene symbol CD28
Gene ID (NCBI) 940
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.