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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD28 Recombinant antibody (83383-7-RR)

    WB analysis using 83383-7-RR (same clone as 83383-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83383-7-RR (CD28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83383-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83383-7-RR

    Affinity and Kinetic Characterization of 83383-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83383-7-RR against Human CD28 were performed. The affinity constant is 2.75 nM.

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CD28 Recombinant antibody, PBS Only

CD28 Uni-rAbTM Recombinant Antibody for ELISA, WB
Cat No. 83383-7-PBS
Clone No.240354D9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

ELISA, WB

Synonyms

Tp44, T-cell-specific surface glycoprotein CD28, CD28 molecule, 240354D9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83383-7-PBS targets CD28 in ELISA, WB applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD28 molecule
Calculated molecular weight 220 aa, 25 kDa
GenBank accession numberBC093698
Gene Symbol CD28
Gene ID (NCBI) 940
ENSEMBL Gene IDENSG00000178562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10747
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.