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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD28 Recombinant antibody

CD28 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240354D9

Cat no : 83383-7-RR

Synonyms

Tp44, T-cell-specific surface glycoprotein CD28, CD28 molecule, 240354D9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD28 Recombinant antibody (83383-7-RR)

    WB analysis using 83383-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83383-7-RR (CD28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83383-7-RR

    Affinity and Kinetic Characterization of 83383-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83383-7-RR against Human CD28 were performed. The affinity constant is 2.75 nM.

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Tested Applications

Positive WB detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83383-7-RR is CD28 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD28 molecule
Calculated molecular weight 220 aa, 25 kDa
Observed molecular weight40-50 kDa
GenBank accession numberBC093698
Gene symbol CD28
Gene ID (NCBI) 940
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).

Protocols

Product Specific Protocols
WB protocol for CD28 antibody 83383-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols