CoraLite®594 Anti-Human CD28 Rabbit Recombinant Antibody
CD28 Recombinant Antibody for FC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
FC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
240354D2
Cat no : CL594-98032
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | human PBMCs |
Recommended dilution
Application | Dilution |
---|---|
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of CL594-98032 is CD28 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Fusion Protein 相同性解析による交差性が予測される生物種 |
Full Name | CD28 molecule |
Calculated molecular weight | 220 aa, 25 kDa |
GenBank accession number | BC093698 |
Gene symbol | CD28 |
Gene ID (NCBI) | 940 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. |
Background Information
CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).
Protocols
Product Specific Protocols | |
---|---|
FC protocol for CL594 CD28 antibody CL594-98032 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |