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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 594 Anti-Human CD29 (TS2/16)

CD29 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

TS2/16

Cat no : CL594-65191

Synonyms

ITGB1, ITB1, Integrin beta-1, Integrin beta 1, GPIIA

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite®594 Anti-Human CD29 (TS2/16) (CL594-65191)

    FC experiment of human PBMCs using CL594-65191

    1x10^6 FC receptor blocked human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD29 (CL594-65191, Clone: TS2/16) (red) or CoraLite®594 Mouse IgG1 Isotype Control (blue). Cells were not fixed. Lymphocytes and monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite®594 Anti-Human CD29 (TS2/16) (CL594-65191)

    FC experiment of human PBMCs using CL594-65191

    1x10^6 FC receptor blocked human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD29 (CL594-65191, Clone: TS2/16) or CoraLite®594 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes and monocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65191 targets CD29 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Calculated molecular weight 88 kDa
GenBank accession numberBC020057
Gene symbol Integrin beta 1
Gene ID (NCBI) 3688
Conjugate CoraLite® Plus 594 Fluorescent Dye
Excitation/Emission maxima wavelengths594 nm / 615 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

Protocols

Product Specific Protocols
FC protocol for CL Plus 594 CD29 antibody CL594-65191Download protocol
Standard Protocols
Click here to view our Standard Protocols