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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody

CD3 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

OKT3

Cat no : 65569-1-MR

Synonyms

CD3E, CD3 epsilon, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, OKT3

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Anti (65569-1-MR)

    FC experiment of human PBMCs using 65569-1-MR

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD19, and 0.25 ug Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (65569-1-MR, Clone:OKT3) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Anti (65569-1-MR)

    FC experiment of human PBMCs using 65569-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (65569-1-MR, Clone: OKT3) (red) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65569-1-MR targets CD3 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human T cells 相同性解析による交差性が予測される生物種
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Calculated molecular weight 207 aa, 23 kDa
GenBank accession numberBC049847
Gene symbol CD3
Gene ID (NCBI) 916
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
FC protocol for CD3 antibody 65569-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols