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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Biotin Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody

CD3 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

Biotin

CloneNo.

OKT3

Cat no : Biotin-65569

Synonyms

CD3E, T3E, TCRE

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Biotin Anti-Human CD3 (OKT3) Mouse IgG2a Recombina (Biotin-65569)

    FC experiment of human PBMCs using Biotin-65569

    1x10^6 human PBMCs were surface stained with 0.25 ug Biotin CD3 Recombinant Antibody (Biotin-65569, Clone: OKT3) or 0.25 ug Isotype Control, and CoraLite®488-conjugated streptavidin. Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Biotin Anti-Human CD3 (OKT3) Mouse IgG2a Recombina (Biotin-65569)

    FC experiment of human PBMCs using Biotin-65569

    1x10^6 human PBMCs were surface stained with 0.25 ug Biotin CD3 Recombinant Antibody (Biotin-65569, Clone: OKT3) and CoraLite®488-conjugated streptavidin (red) or 0.25 ug Isotype Control and CoraLite®488-conjugated streptavidin (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-65569 targets CD3 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human T cells 相同性解析による交差性が予測される生物種
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Calculated molecular weight 207 aa, 23 kDa
GenBank accession numberBC049847
Gene symbol CD3
Gene ID (NCBI) 916
Conjugate Biotin
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
FC protocol for Biotin CD3 antibody Biotin-65569Download protocol
Standard Protocols
Click here to view our Standard Protocols