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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PerCP-Cyanine5.5 Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody

CD3 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

PerCP-Cyanine5.5 Fluorescent Dye

CloneNo.

UCHT1

Cat no : CPY5-65570

Synonyms

CD3E, CD3 epsilon, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, T3E

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using PerCP-Cyanine5.5 Anti-Human CD3 (UCHT1) Mouse IgG2 (CPY5-65570)

    FC experiment of human PBMCs using CPY5-65570

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD19, and 5 ul PerCP-Cyanine5.5 Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody (CPY5-65570, Clone:UCHT1) or unstained. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using PerCP-Cyanine5.5 Anti-Human CD3 (UCHT1) Mouse IgG2 (CPY5-65570)

    FC experiment of human PBMCs using CPY5-65570

    1x10^6 human PBMCs were surface stained with 5 ul PerCP-Cyanine5.5 Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody (CPY5-65570, Clone:UCHT1)(red), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CPY5-65570 targets CD3 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor 相同性解析による交差性が予測される生物種
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Calculated molecular weight 207 aa, 23 kDa
GenBank accession numberBC049847
Gene symbol CD3
Gene ID (NCBI) 916
Conjugate PerCP-Cyanine5.5 Fluorescent Dye
Excitation/Emission maxima wavelengths482 nm / 690nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
FC protocol for PerCP-Cyanine5.5 CD3 antibody CPY5-65570Download protocol
Standard Protocols
Click here to view our Standard Protocols