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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD3 Gamma Monoclonal antibody, PBS Only

CD3 Gamma Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E9G7

Cat no : 60347-1-PBS

Synonyms

CD3 gamma, CD3G, T cell receptor T3 gamma chain, T3G

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD3 Gamma Monoclonal antibody (60347-1-Ig)

    WB analysis of Jurkat using 60347-1-Ig (same clone as 60347-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60347-1-Ig (CD3 gamma antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60347-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD3 Gamma Monoclonal antibody (60347-1-Ig)

    IHC staining of human tonsillitis using 60347-1-Ig (same clone as 60347-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60347-1-Ig (CD3 gamma antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 60347-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD3 Gamma Monoclonal antibody (60347-1-Ig)

    IHC staining of human tonsillitis using 60347-1-Ig (same clone as 60347-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60347-1-Ig (CD3 gamma antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 60347-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60347-1-PBS targets CD3 Gamma in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD3 Gamma fusion protein Ag15502 相同性解析による交差性が予測される生物種
Full Name CD3g molecule, gamma (CD3-TCR complex)
Calculated molecular weight 182 aa, 20 kDa
Observed molecular weight 20-25 kDa
GenBank accession numberBC113830
Gene symbol CD3 Gamma
Gene ID (NCBI) 917
RRIDAB_2881456
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.