CD3 Gamma Monoclonal antibody
CD3 Gamma Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2E9G7
Cat no : 60347-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
60347-1-Ig targets CD3 Gamma in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD3 Gamma fusion protein Ag15502 相同性解析による交差性が予測される生物種 |
| Full Name | CD3g molecule, gamma (CD3-TCR complex) |
| Calculated molecular weight | 182 aa, 20 kDa |
| Observed molecular weight | 20-25 kDa |
| GenBank accession number | BC113830 |
| Gene symbol | CD3 Gamma |
| Gene ID (NCBI) | 917 |
| RRID | AB_2881456 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD3 Gamma antibody 60347-1-Ig | Download protocol |
| IHC protocol for CD3 Gamma antibody 60347-1-Ig | Download protocol |
| FC protocol for CD3 Gamma antibody 60347-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Sci Metformin changes the immune microenvironment of colorectal cancer in patients with type 2 diabetes mellitus. | ||
J Breast Cancer Metformin Alters Tumor Immune Microenvironment, Improving the Outcomes of Breast Cancer Patients With Type 2 Diabetes Mellitus | ||
J Anus Rectum Colon Reduced Abundance of Phocaeicola in Mucosa-associated Microbiota Is Associated with Distal Colorectal Cancer Metastases Possibly through an Altered Local Immune Environment |
