CoraLite®594-conjugated CD3 Gamma Monoclonal antibody
CD3 Gamma Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
IF-P
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2E9G7
Cat no : CL594-60347
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-60347 targets CD3 Gamma in IF-P applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD3 Gamma fusion protein Ag15502 相同性解析による交差性が予測される生物種 |
| Full Name | CD3g molecule, gamma (CD3-TCR complex) |
| Calculated molecular weight | 182 aa, 20 kDa |
| Observed molecular weight | 20-25 kDa |
| GenBank accession number | BC113830 |
| Gene symbol | CD3 Gamma |
| Gene ID (NCBI) | 917 |
| RRID | AB_2919921 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 CD3 Gamma antibody CL594-60347 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
