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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody, PBS Only

CD30 Ligand/TNFSF8 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241831D9

Cat no : 98253-1-PBS

Synonyms

TNFSF 8, CD30 L, TNFSF8, 241831D9, CD30LG

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant A (98253-1-RR)

    FC experiment of human PBMCs using 98253-1-RR (same clone as 98253-1-PBS)

    1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. This data was developed using the same antibody clone with 98253-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant A (98253-1-RR)

    FC experiment of human PBMCs using 98253-1-RR (same clone as 98253-1-PBS)

    1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98253-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98253-1-PBS targets CD30 Ligand/TNFSF8 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (ligand) superfamily, member 8
Calculated molecular weight 234 aa, 26 kDa
GenBank accession numberBC093630
Gene symbol TNFSF8
Gene ID (NCBI) 944
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD30 Ligand (CD30L), also named as TNFSF8 or CD153, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) superfamily (PMID: 8391931). It is expressed on the surface of activated T cells, B cells, monocytes, macrophages, eosinophils, neutrophils, and mast cells (PMID: 26090498). CD30L interacts with its receptor, CD30, which is a cell surface protein expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin lymphoma and anaplastic large cell lymphomas (PMID: 28885612). The CD30/CD30L signaling is involved in pleiotropic downstream effects including differentiation, cell survival and death, NFkB activation, and production of cytokines (PMID: 38534788).