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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant A (98253-1-RR)

    FC experiment of human PBMCs using 98253-1-RR

    1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant A (98253-1-RR)

    FC experiment of human PBMCs using 98253-1-RR

    1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody (98253-1-RR, Clone: 241831D9) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98253-1-RR

    Affinity and Kinetic Characterization of 98253-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98253-1-RR against Human CD30 Ligand/TNFSF8 were performed. The affinity constant is below 1 pM.

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Anti-Human CD30 Ligand/TNFSF8 Rabbit Recombinant Antibody

CD30 Ligand/TNFSF8 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98253-1-RR
Clone No.241831D9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

TNFSF 8, CD30 L, TNFSF8, 241831D9, CD30LG

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive FC detected inPMA and ionomycin treated human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98253-1-RR targets CD30 Ligand/TNFSF8 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (ligand) superfamily, member 8
Calculated molecular weight 234 aa, 26 kDa
GenBank accession numberBC093630
Gene Symbol TNFSF8
Gene ID (NCBI) 944
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP32971
Storage Buffer PBS with 0.09% sodium azide , pH 7.3
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD30 Ligand (CD30L), also named as TNFSF8 or CD153, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) superfamily (PMID: 8391931). It is expressed on the surface of activated T cells, B cells, monocytes, macrophages, eosinophils, neutrophils, and mast cells (PMID: 26090498). CD30L interacts with its receptor, CD30, which is a cell surface protein expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin lymphoma and anaplastic large cell lymphomas (PMID: 28885612). The CD30/CD30L signaling is involved in pleiotropic downstream effects including differentiation, cell survival and death, NFkB activation, and production of cytokines (PMID: 38534788).

Protocols

Product Specific Protocols
FC protocol for CD30 Ligand/TNFSF8 antibody 98253-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols